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. 2018 Aug 1;115(33):E7844–E7853. doi: 10.1073/pnas.1806040115

Fig. 1.

Fig. 1.

Effects of Ala substitution of GA4-interacting amino acids of OsGID1 on its activity in planta. (A) Amino acids are categorized by their commonality among GID1s and GID1-like CXEs in SI Appendix, Fig. S2: such as type I (orange), shared by all GID1s and GID1-like CXEs; type II (dark blue), shared by all GID1s but not CXEs; type III (red), shared by seed plants and ferns but not Selaginella; type IV (green), shared by seed plants but not nonseed plants; and type V (sky blue), not conserved among seed plants. Polar and nonpolar interactions are indicated by arrows and half circles, respectively. The C6-COOH, C3-OH, and C2 positions of GA4 are marked in yellow. (BD) Rescued phenotypes by transformed mGID1s carrying mutated amino acids interacting with C6-COOH (B) and C3-OH (C), and involved in nonpolar interaction (D). (Scale bars: 5 cm.)