Skip to main content
. 2018 Jul 30;115(33):8418–8423. doi: 10.1073/pnas.1808426115

Fig. 2.

Fig. 2.

Minimal therapeutic effects of hemin on symptoms of DSS-induced colitis in Lyz2-cre; Spicflox/flox mice. Spicflox/flox and Lyz2-cre; Spicflox/flox mice intraperitoneally injected with 1 mg hemin or vehicle on days −2 and −1 of DSS administration and administered with 1.5% DSS for 7 d (n = 8–9 per group) (A, E, and F) or 11 d (n = 5–8 per group) (B–D). (A) Weight changes. *P < 0.05, ****P < 0.001, #P < 0.05, $P < 0.05, $$P < 0.01, &P < 0.05, &&P < 2 × 10−5. (B and C) Bleeding score (B) and stool score (C). *P < 0.05, **P < 0.01, ***P < 0.005, #P < 0.05, $P < 0.05, &P < 0.05, *P denotes significance between heme-treated control and Spic mutant mice, #P denotes significance between vehicle-injected control and Spic mutant mice, $P denotes significance between vehicle-injected and heme-injected Spic mutant mice, and &P denotes significance between vehicle-injected and heme-injected control mice (A–C). (D) Colon length. ***P < 0.005, ****P < 0.001. (E) Representative colon sections (Upper) and histological score (Bottom) of the colons. *P < 0.05, ***P < 0.005. (F) Frequency (Left) and number (Right) of IFN-γ–, IL-17–, and IL-10–producing CD4+ T cells. *P < 0.05, **P < 0.01.