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. 2018 Jul 30;115(33):8418–8423. doi: 10.1073/pnas.1808426115

Fig. 5.

Fig. 5.

Spi-C suppresses the expression of a subset of TLR4-dependent genes by interacting with IRF5. (A) Enrichment analysis of the upstream transcriptional regulators of genes elevated in Spic−/− BMDMϕs. (B–D) Coimmunoprecipitation (IP) assay with anti-His (B) and anti–NF-κB p65 (C and D) antibodies for immunoprecipitation and the indicated antibodies for immunoblotting. (E–G) ChIP assay for the Il6 promoter of the NF-κB–binding site was performed with anti-His (E), anti-IRF5 (F), and anti–NF-κB p65 (G) antibodies. All graphs show the mean ± SD. Data are representative of two independent experiments. **P < 0.01. n.s., not significant. (H and I) ChIP assay for the NF-κB site of the Il6 promoter and Il1a promoter was performed with anti-IRF5 (H) and anti–NF-κB p65 (I) antibodies using control and Spic−/− BMDMϕs stimulated with LPS following pretreatment with hemin for 18 h. Graphs show the means ± SD. Data are representative of two independent experiments. *P < 0.05, ****P < 0.001.