Fig. 1.

Mutations in M6 induce invasion of Ras^V12 clones. (A) Clones (GFP labeled) of the indicated genotypes were fixed in the cephalic complex (CC, Top) to show overall clone size and in the VNC (Bottom) to show invasion (n > 150 of each genotype). M6^−/− notation refers to the M6^W186* allele. (Scale bar: Top, 200 um; Bottom, 50 um.) (B) Quantification of the percentage of larvae that show invasion into the VNC. Three biological replicates of 50 larva for each genotype were scored for invasion. Data are presented as mean ± SD. (C and D) Ras^V12;M6^−/− clones in the eye disk stained for common hallmarks of invasive tumors including MMP1 expression and JNK activation. Dashed lines represent outlines of the eye disk. (Scale bar: 100 um.) n = 5.