Fig. 4.

Cno is necessary for RhoA-induced apical delamination and invasion in Ras^V12; M6^−/− clones. (A) Ras^V12 and Ras^V12; M6^−/− eye-disk clones were stained for Cno (n = 10 discs of each genotype). (Scale bar: 50 um.) (Right) Boxed area enlarged. (Scale bar: Right, 50 um.) (B) RhoA mean stain intensity was quantified for clones of the indicated genotypes (n = 59 Ras^V12 clones, 116 Ras^V12; M6^−/− clones, and 113 Cno RNAi; Ras^V12, M6^−/− clones). Significance was calculated using a Mann–Whitney U test. **P < 0.01. Data presented as mean with 95% CI. (C) Quantification of the localization of clones of indicated genotypes (n = 102 Ras^V12; M6^−/− clones and 90 Cno RNAi; Ras^V12; M6^−/− clones). Statistical significance was analyzed using χ² analysis. **P < 0.01. (D) The VNCs from flies of the indicated genotypes were dissected and scored for the presence of GFP+ invasive cells (n = 31 Ras^V12; M6^−/− clones and 35 Cno RNAi; Ras^V12; M6^−/− clones). Significance was calculated using Fisher’s exact text. **P < 0.01.