Fig. 4.

AMH signaling is necessary and sufficient for OSX expression in the MD mesenchyme. (A and B) Immunofluorescence for OSX on paraffin sections of mesonephroi at E14.5. OSX is not expressed in Amhr2^Z/Cre males, indicating AMH signaling is required for OSX expression in the male MD. (C–F) β-Galactosidase staining of Osx^Z/+ and Osx^Z/+; Mt-hAMH female reproductive tracts. Activation of the AMH signaling pathway in the female reproductive tract is sufficient to induce expression of Osx-lacZ in the MD. MD and WD epithelium outlined with a dashed line. b, bladder; MD, Müllerian duct; o, ovary; WD, Wolffian duct. [Scale bars: 100 µm (A, B, E, and F); 500 µm (C and D).]