Fig. 6.
Progression of MD regression is delayed in OSXZ/Z-null mice. (A–F) Whole-mount β-galactosidase staining of OsxZ/+ (A–D) and OsxZ/Z (E–H) male reproductive tracts. (A and E) E15.5 OsxZ/Z males have intact MDs that appear thicker than OsxZ/+. (B and F) E16.5 OsxZ/Z males have longer lengths of MD remnants in comparison with OsxZ/+ males. (C and G) E17.5 MD tissue remnants are observed near the testis in OsxZ/Z males. (D and H) E18.5 OsxZ/Z regression defects are resolved and appear similar to OsxZ/+ males. (Scale bar: 500 µm.) Three or more of each genotype were observed for each developmental time point.