Table 2. Specific activity (U mg–1) of WT MAO-N and evolved variants in addition to mutants LG-F-B6 and LG-F-B5 (marked in bold), which were created by deconvoluting LG-F-B7.
Entry | Mutations | 1 | 2 | 3 |
WT MAO-N | 0 | 0 | 0.13 ± 0.01 | |
LG-F-G6 | W230R/W430C | 0.108 ± 0.001 | 0.23 ± 0.02 | 0.04 ± 0.002 |
LG-I-D11 | W230R/W430C/C214L | 0.66 ± 0.01 | 0.52 ± 0.05 | 0.23 ± 0.02 |
LG-F-B7 | W230I/T354S/W430R | 0.02 ± 0.001 | 0.30 ± 0.01 | 0.02 ± 0.003 |
LG-J-B4 | W230I/T354S/W430R/M242R/Y365V | 0.018 ± 0.001 | 0.67 ± 0.03 | 0.03 ± 0.003 |
LG-F-B6 | W230I/W430R | 0.014 ± 0.001 | 0.22 ± 0.02 | 0.016 ± 0.004 |
LG-F-B5 | T354S | 0.025 ± 0.001 | 0 | 0.09 ± 0.01 |