Table 3. Kinetic characterization of the best MAO-N variants, mutant LG-F-B7 and deconvolutant LG-F-B6.
Enzyme | Substrate | K m (mM) | k cat (min–1) | k cat/Km (min–1 mM–1) |
LG-I-D11 | 1 | 1.12 ± 0.09 | 61.77 ± 1.41 | 55.15 |
LG-J-B4 | 2 | 1.25 ± 0.06 | 46.02 ± 0.60 | 36.81 |
LG-F-B7 | 2 | 1.42 ± 0.07 | 37.40 ± 0.72 | 26.34 |
LG-F-B6 | 2 | 1.96 ± 0.12 | 21.35 ± 0.43 | 10.89 |