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Chrysin did not increase p21 mRNA or p21 protein levels in p53-null and p53-mutant cells. (A) PC3 cells and HaCaT cells were treated with ADR (0.3 μM) or chrysin (40 μM) for 8 h. Cell lysates were immunoblotted with the indicated antibodies. (B) PC3 cells and HaCaT cells were treated with ADR (0.3 μM) or chrysin (40 μM) for 8 h. The expression of each gene was assessed by qPCR.
