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. 2018 May 31;23(6):1327. doi: 10.3390/molecules23061327

Figure 3.

Figure 3

Effect of graphene oxide (GO) and reduced graphene oxide (rGO) with extract of black rice with giant embryo (BRGEx) on phosphorylated H2AX protein levels in B3 cells. B3 cells were pretreated with BRGEx (10 µg/mL) in the presence or absence of GO (10 µg/mL) (A,B) or rGO (10 µg/mL) (C,D) for 48 h, then 1 mM of MGO (A,C) and 100 µM of H2O2 (B,D) was added to the cells for 2 h. Phosphorylated h2AX protein levels were determined using Western blotting, and β-actin was used as loading control.