Figure 4.

Effect of graphene oxide (GO) and reduced graphene oxide (rGO) with or without soybean extract (SBx) on phosphorylated H2AX protein levels in B3 cells. B3 cells were pretreated with SBx (10 µg/mL) in the presence of GO (10 µg/mL) (A,B) or rGO (10 µg/mL) (C,D) for 48 h then 1 mM of MGO (A,C) and 100 µM of H₂O₂ (B,D) was added to the cells for 2 h. Phosphorylated H2AX protein levels were determined using Western blotting, and β-actin was used as the loading control.