Table 1.

UPLC gradient method.

Time, min.	% A (ACN:MeOH = 7:3)	% B (Water)	Flow Rate (mL/min)
Initial	75	25	0.5
0.6	75	25	0.5
6.5	95.1	4.9	0.5
7.5	100	0	0.5
13.6	100	0	0.5
14.1	75	25	0.5
16.6	75	25	0.5