Figure 5.

The effects of garcinol on TRAIL sensitization in cancer and normal cells. (A) ACHN, A498 (renal carcinoma), A549 (lung carcinoma) and SK-Hep1 (liver carcinoma) cells were treated with 2 μM garcinol and/or 50 ng/mL TRAIL for 24 h. Levels of apoptosis were assessed by flow cytometry, and western blot showing the PARP and actin; (B) Cells were treated with 0.5–2 μM garcinol for 24 h. The related levels of proteins were detected by western blot using indicated antibody; (C) Caki, masangial cells (MC) and TCMK-1 cells were treated with 2 μM garcinol and/or 50 ng/mL TRAIL for 24 h. Cell morphology was explored using an interference optical microscope. Levels of apoptosis were assessed by flow cytometry. * p < 0.01 compared to the control.