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. 2018 Jul 12;23(7):1700. doi: 10.3390/molecules23071700

Figure 3.

Figure 3

Inter-individual variability of the complement activation by Doxil in human plasma. Human plasma anticoagulated with hirudin was collected from 20 healthy donor volunteers and tested in vitro. (A) Plasma from individual donors was spiked with controls or Doxil and incubated for 30 min. The concentration of Doxil was 0.4 mg/mL of doxorubicin and was constant between all samples. Complement activation was assessed by ELISA measuring levels of human iC3b. Each bar shows mean response and standard deviation (n = 3); (B) Stimulation index was calculated for each donor as described in the materials and methods. Donors were grouped into three categories according to the individual magnitude of the complement activation: SI ≤ 2 was assigned as low response, SI 2–6 was assigned as medium response, and SI ≥ 6 was assigned as high response; (C) Regression analysis shows no linear correlation between complement activation by Doxil and CVF in individual plasma samples (p = 0.2112 and r = 0.08521; F = 1.677). SI—stimulation index; CVF—cobra venom factor; NC—negative control; PC—positive control (CVF); D—donor.