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. 2018 Jun 1;23(6):1331. doi: 10.3390/molecules23061331

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Isolation and purification of glycosphingolipids from Rhizopus species. (B) High performance thin layer chromatography (HPTLC) of neutral glycosphingolipid fractions by silica gel column chromatography. P. CMH standard. Lane 1: Methanol fraction from R. stolonifer grown in potato dextrose broth (PDB). Lane 2: Methanol fraction from R. stolonifer grown in yeast extract-peptone-dextrose growth medium (YEPD). Lane 3: Methanol fraction from R. microsporus grown in PDB. Lane 4: Methanol fraction from R. microspores grown in YEPD. Solvent system: Chloroform/methanol/2 M NH₄OH (40:10:1 v/v/v). Detection: iodine vapor and orcinol-sulfuric acid reagent.