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. 2018 Jul 13;23(7):1711. doi: 10.3390/molecules23071711

Figure 2.

Figure 2

Effects of LA on the production and mRNA expression of TSLP in HMC-1 cells. (A) HMC-1 cells (3 × 105) were pretreated with LA at concentrations of 4 to 400 μg/mL for 1 h, and then stimulated by PMA plus A23187 for 7 h. The TSLP levels in the supernatant were assessed using ELISA method. (B) HMC-1 cells (1 × 106) were stimulated by PMA plus A23187 for 5 h. The levels of TSLP mRNA expression were measured using qPCR method. (C) HMC-1 cells (3 × 105) were pretreated with LA at the doses of 4 to 400 μg/mL for 1 h, and then stimulated with PMA plus A23187 for 7 h. Cell viability was determined with an MTT assay. B, PBS-treated cells; C, PBS-treated, and then PMA plus A23187-stimulated cells. Each datum represents the mean ± S.E.M. of three independent experiments. * p < 0.05; significantly different from the control (PBS-treated, and then PMA plus A23187-stimulated cells) value.