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Total melanin content assay—the tested compound was added to a final concentration of 30 μM of B16-F10 melanoma grown cells. The compounds genistein (4), duartin (22), and (2S)-naringenin (25) increased the melanin content. The inhibitory effect of melanogenesis was found in cajanin (9) and (6aR,11aR)-3,8-dihydroxy-9-methoxy pterocarpan (27). Oxyresveratrol (Oxy) was used as a positive control. (C) is the negative control to which 0.5% dimethyl sulfoxide (DMSO) was added.
