Table 3.
Murine tyrosinase inhibitory activity and melanogenesis of flavonoids from Dalbergia parviflora.
| No. | Isoflavones | Murine Tyrosinase Inhibition, % (at 200 μM) | 15 μM | 30 μM | ||
|---|---|---|---|---|---|---|
| % Melanin Content | % Cell Viability | % Melanin Content | % Cell Viability | |||
| 1 | Formononetin | - | 133.8 ± 2.7 | 96.0 ± 3.3 | 155.3 ± 15.4 | 92.8 ± 9.4 |
| 2 | Calycosin | - | 142.8 ± 8.2 | 99.1 ± 9.2 | 196.6 ± 6.4 | 101.3 ± 1.9 |
| 3 | Biochanin A | - | 165.2 ± 5.9 | 103.4 ± 4.5 | 168.0 ± 5.1 | 102.2 ± 4.8 |
| 4 | Genistein | 9.9 ± 3.1 | 165.1 ± 1.6 | 128.2 ± 3.5 | 191.7 ± 13.9 | 93.8 ± 5.7 |
| 5 | Khrinone B | 10.1 ± 3.0 | 84.1 ± 5.3 | 95.1 ± 5.4 | 34.7 ± 12.3 | 62.3 ± 15.1 |
| 6 | 3′-O-Methylorobol | - | 170.1 ± 10.5 | 106.6 ± 6.2 | 215.7 ± 15.7 | 101.5 ± 2.7 |
| 7 | Khrinone C | - | 107.6 ± 0.8 | 93.3 ± 2.0 | 154.0 ± 9.9 | 93.4 ± 2.0 |
| 8 | Tectorigenin | 10.2 ± 2.9 | 127.6 ± 2.8 | 129.7 ± 3.4 | 152.1 ± 4.7 | 119.3 ± 1.3 |
| 9 | Cajanin | 9.2 ± 1.5 | 65.8 ± 7.5 | 101.5 ± 6.6 | 61.6 ± 8.9 | 107.2 ± 3.3 |
| No. | Isoflavanones | |||||
| 10 | (3R)-7,3′-Dihydroxy-4′-methoxy-isoflavanone | - | 95.1 ± 3.0 | 94.9 ± 3.9 | 95.2 ± 2.7 | 96.5 ± 3.9 |
| 11 | Onogenin | - | 133.5 ± 4.6 | 100.0 ± 5.0 | 169.8 ± 15.4 | 92.0 ± 2.0 |
| 12 | Dalparvin | - | 133.8 ± 9.7 | 117.1 ± 8.9 | 199.6 ± 12.1 | 99.7 ± 6.7 |
| 13 | Dalparvin B | 9.8 ± 2.8 | 102.0 ± 2.7 | 82.3 ± 5.4 | 99.1 ± 2.3 | 74.1 ± 6.4 |
| 14 | (3S)-Sativanone | 9.5 ± 1.6 | 124.2 ± 4.3 | 106.7 ± 10.6 | 127.6 ± 6.0 | 64.3 ± 23.7 |
| 15 | (3R,S)-3′-O-Methylviolanone | - | 143.3 ± 8.4 | 74.2 ± 7.8 | 157.2 ± 11.5 | 72.9 ± 6.2 |
| 16 | (3R,S)-Kenusanone G | - | 132.6 ± 5.1 | 124.0 ± 1.7 | 158.5 ± 13.5 | 102.2 ± 2.8 |
| 17 | (3S)-Secundiflorol H | - | 117.6 ± 17.7 | 109.4 ± 6.6 | 146.4 ± 6.1 | 102.7 ± 4.1 |
| 18 | Dalparvin A | - | 132.0 ± 4.4 | 105.4 ± 4.8 | 184.2 ± 10.6 | 100.4 ± 7.1 |
| No. | Isoflavans | |||||
| 19 | (3R)-Vestitol | 12.4 ± 3.3 | 186.1 ± 1.6 | 142.1 ± 3.0 | 232.0 ± 9.3 | 119.7 ± 4.1 |
| 20 | (3R)(+)-Mucronulatol | 12.8 ± 2.1 | 178.6 ± 15.9 | 103.6 ± 6.9 | 263.7± 8.2 | 102.5 ± 2.7 |
| 21 | (3RS)-3′-Hydroxy-8-methoxy Vestitol | 11.4 ± 2.5 | 97.0 ± 6.3 | 102.9 ± 1.0 | 36.3 ± 0.1 | 62.4 ± 3.2 |
| 22 | Duartin | - | 301.1 ± 2.3 | 139.9 ± 7.5 | 326.8 ± 2.1 | 94.4 ± 2.8 |
| 23 | (3S)-8-Demethylduartin | - | 87.8 ± 2.0 | 93.5 ± 3.0 | 72.7 ± 4.3 | 98.3 ± 5.4 |
| No. | Flavanones | |||||
| 24 | (2S)-Liquiritigenin | 13.2 ± 2.7 | 102.9 ± 5.5 | 98.9 ± 10.7 | 124.5 ± 2.0 | 100.0 ± 3.5 |
| 25 | (2S)-Naringenin | - | 132.5 ± 14.5 | 94.3 ± 2.0 | 152.1 ± 3.8 | 98.1 ± 0.5 |
| 26 | Alpinetin | - | 89.7 ± 0.4 | 77.0 ± 12.7 | 92.3 ± 1.6 | 77.1 ± 10.2 |
| No. | Pterocarpan | |||||
| 27 | (6aR,11aR)-3,8-Dihydroxy-9-methoxy pterocarpan | 29.2 ± 2.9 | 41.9 ± 0.4 | 105.7 ± 4.9 | 53.5 ± 0.4 | 14.5 ± 0.9 |
| Others | ||||||
| Control | Kojic acid | 73.8 ± 1.0 | 68.9 ± 5.2 | 98.9 ± 4.7 | 74.2 ± 5.8 | 99.6 ± 2.3 |
| Control | Oxyresveratrol | 99.4 ± 1.0 | 24.3 ± 1.1 | 117.2 ± 2.2 | 28.4 ± 1.7 | 118.1 ± 5.1 |
-: no inhibition at 200 μM.