Figure 3.

Autoradiogram of reaction products obtained from MAT-catalyzed reactions. Lochnericine (lane 1), hörhammericine (lane 2), or a minovincinine-containing root extract (lane 3) were used as substrates. The reaction products were extracted and chromatographed as described in “Material and Methods.” The structures of the respective acetylated products are shown. Although no acetylated product was observed when the supplied substrate was lochnericine, its structure is included to show the requirement for the hydroxyl group at position 19 for enzyme activity.