Figure 4.

Northern blots of total RNA isolated from Madagascar periwinkle hairy roots, roots, stems, leaves, flower petals, or etiolated seedlings, respectively. Blots were probed with the DAT ORF fragment and a 423-bp HindIII fragment of MAT::pBluescript under high stringency conditions. RNA was quantified by ethidium bromide staining and 20 μg of total RNA per sample was electrophoresed on a 7% (v/v) formaldehyde agarose gel. RNA was transferred to nitrocellulose for hybridization.