Table 1.
Sequencing readout for 5hmU‐modified ODN1.
| Protocol[a] | base | %T[b] | %C[b] | %other[b] |
|---|---|---|---|---|
| Steps 1–4 | 5hmU1 | 50.4±3.0 | 39.4±3.4 | 9.9±0.4 |
| 5hmU2 | 65.1±1.4 | 30.3±1.5 | 4.6±0.3 | |
| T[c] | 98.2±0.3 | 1.4±0.3 | <1 | |
| Steps 1, 3, and 4 (no‐oxidation control) |
5hmU1 | 97.3±2.3 | 2.2±1.0 | <1 |
| 5hmU2 | 95.9±1.1 | 2.7±0.1 | 1.3±1.2 | |
| T[c] | 98.3±1.1 | 1.2±1.0 | <1 |
[a] Steps as shown in Figure 1. Step 3 was carried out at 37 °C with 10 mm MgSO4 and dNTP mix (final concentrations: 250 μm for dCTP, dGTP, and TTP and 500 nm for dATP) using Bst DNA Polymerase, Large Fragment. See Supporting Information for details. [b] The proportion of reads giving T, C, or other signal (that is, A, G, insertion, and deletion) at the 5hmU‐modified sites over all reads. Mean±SD values of technical triplicates (at least two data out of three were obtained in coverage depth of greater than 1000×) are shown. [c] Mean values for seven proximal Ts.