Figure 4.

Time course of lipid peroxidation and other events during aluminum treatment in pea roots. Pea seedlings were treated with (●) or without (○) 10 μm aluminum in 100 μm CaCl₂ (pH 4.75) for up to 24 h. Roots were harvested at selected time points for analyses of aluminum content (A), lipid peroxidation (B), callose content (C), root elongation, (D), or the loss of plasma membrane integrity (E), as described in “Materials and Methods.” Although the elongation data of control roots is shown from 0 to 12 h (E), the control roots continued to elongate until 24 h with an average length of 24 ± 1 mm. All data show the means ± se of a total of three replicates from two independent experiments, each replicate comprising four 10-mm root apices (A, B, and E), one 10-mm root apex (C), or six roots (D).