Table 1.
Percentage inhibition of GFP fluorescence and the reduction of OD by the synthesized lactams against P. aeruginosa MH602.
| QSI Activity against P. aeruginosa MH602 | |||
|---|---|---|---|
| Concentration (µM) | |||
| Compound | 250 | 125 | 62.5 |
| 9 | b 71.4 ± 1.0 | a 51.0 ± 1.8 | a 47.8 ± 0.8 |
| 10 | b 68.0 ± 0.5 | a 50.6 ± 0.5 | a 44.3 ± 7.0 |
| 11 | b 62.1 ± 3.4 | a 39.4 ± 2.5 | a 38.2 ± 5.0 |
| 12 | b 83.0 ± 2.8 | a 82.0 ± 2.6 | a 69.4 ± 0.3 |
| 13 | b 78.3 ± 2.3 | a 74.6 ± 0.8 | a 65.7 ± 0.3 |
| 14 | a 58.0 ± 3.3 | a 47.8 ± 1.3 | a 35.2 ± 5.7 |
| 15 | a 35.7 ±1.8 | a 25.92 ± 2.5 | a 15.35 ± 0.8 |
| 16 | a 19.8 ± 3.3 | a 13.25 ± 3.9 | a 6.85 ± 1.8 |
| 19 | a 80.4 ± 1.1 | a 79.5 ± 0.6 | a 78.1 ± 1.6 |
| 20 | a 58.82 ± 5.8 | a 36.97 ± 3.2 | a 22.82 ± 3.2 |
| 21 | a 17.23 ± 3.0 | a 13.5 ± 3.9 | a 9.27 ± 2.3 |
| 22 | a 76.4 ± 1.2 | a 60.5 ± 0.5 | a 48.2 ± 2.0 |
| 23 | b 81.7 ± 0.1 | a 78.7 ± 0.5 | a 76.5 ± 0.4 |
| 24 | b 80.7 ± 3.0 | a 77.5 ± 4.1 | a 69.7 ± 0.9 |
| 25 | a 55.8 ± 1.2 | a 54.2 ± 2.8 | a 37.1 ± 1.6 |
| 26 | a 28.17 ± 3.1 | a 16.25 ± 1.9 | a 9.23 ± 1.1 |
| 27 | a 13.33 ± 3.0 | a 6.2 ± 3.5 | NA |
| 29 | a 42.55 ± 3.3 | a 26.15 ± 3.1 | a 13.55 ± 2.6 |
| 30 | a 74.0 ± 1.7 | a 43.1 ± 1.1 | a 32.1 ± 3.5 |
| 31 | a 43.6 ± 6.5 | a 40.2 ± 6.2 | a 33.5 ± 2.8 |
| 32 | a 52.5 ± 6.2 | a 41.7 ± 4.1 | NA |
| 33 | a 32.3 ± 4.6 | a 3.5 ± 5.8 | NA |
| 34 | a 54.2 ± 5.9 | a 30.9 ± 6.0 | a 25.3 ± 1.0 |
| 35 | a 53.5 ± 5.6 | a 28.7 ± 3.2 | a 14.4 ± 4.7 |
| 36 | a 33.5 ± 0.5 | a 20.8 ± 2.1 | NA |
| 37 | a 37.1 ± 2.1 | a 29.1 ± 2.7 | a 4.0 ± 1.88 |
| 38 | a 30.0 ± 3.9 | a 21.5 ± 1.6 | NA |
| 39 | a 41.4 ± 2.8 | a 32.2 ± 3.7 | a 16.7 ± 3.9 |
| 40 | a 36.0 ± 3.0 | a 31.7 ± 2.0 | a 4.6 ± 0.7 |
| 41 | a 53.6 ± 3.1 | a 32.7 ± 4.0 | a 16.9 ± 5.5 |
| 42 | a 34.5 ± 2.0 | a 30.2 ± 4.4 | a 12.2 ± 2.9 |
| d TP-5 | a 34.4 ± 5.2 | a 30.4 ± 3.2 | a 2.3 ± 0.3 |
| d Fu-30 (5) | c 84.8 ± 2.9 | c 82.5 ± 2.5 | c 74.4 ± 3.6 |
a Bacterial growth inhibition ≤15%; b growth inhibition between 15 and 30%; c growth inhibition greater than 30%; d positive control; ± the standard deviation of the mean for three independent experiments. In each independent experiment, compounds were tested in triplicate. NA = no activity and no growth inhibition (supplementary materials Table S1).