The effect of different compounds, prosopilosidine (1), isoprosopilosidine (2), juliprosine (3), prosopilosine (4), isoprosopilosine (5), juliprosopine (6) and Amphotericin B (Amp B) on cell viability. HepG2 cells were seeded on 96-well plates and were treated with 1, 10, 20, and 50 µg/mL of the test compounds or with vehicle only for 24 h. The MTS assay for cell viability and proliferation was performed with the CellTiter 96®AQueous Non-Radioactive Cell Proliferation Assay Kit (Promega). Data are shown as mean ± SEM and were analyzed with ANOVA. #
p < 0.001 is considered statistically significant compared to the untreated cells (100%).