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AA impaired autophagic influx. (A,B) Red LC3 puncta are greatly induced in cells after treatment with AA or rapamycin. Co-localized green fluorescence is significantly increased in AA-treated cells (n = 20) as compared with rapamycin-treated cells (n = 20). Student’s t test p-value: *** p < 0.01. (C) AA did not affect the fluorescent signals of lysotrackers. In contrast, Bafilomycin A1, the V-ATPase inhibitor completely abolishes the fluorescence.
