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. 2018 Jul 9;23(7):1663. doi: 10.3390/molecules23071663

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Extraction and fractionation of bioactive components from a 30% ethanol extract of Gentiana lutea L. root (GL). The 30% ethanol extract of the GL roots was fractionated using chloroform, ethyl acetate, and butanol. The butanol fraction was further fractionated by column chromatography using octadecylsilyl (ODS) gel and was eluted with methanol in H₂O (1/4, v/v) to obtain five fractions (GL-B-01-05). Fraction GL-B-03 was subjected to RP-18 column chromatography and was eluted with acetonitrile (ACN) in H₂O and 0.05% trifluoroacetic acid (TFA) in a step-gradient manner (10–40%), finally producing a bioactive compound. Abbreviations: fr., fraction; HPLC, high-performance liquid chromatography.