Figure 3.

The effects of loganic acid on the mRNA expression of adipogenesis-related genes during adipocyte differentiation of 3T3-L1 cells. The cells were treated with 2, 10, or 50 μg/mL of loganic acid (LA2, LA10, or LA50). (A) The expression levels of mRNA of 8 genes were calculated quantitatively by reverse-transcription real-time PCR using gene-specific primers and were then normalized to the Gapdh mRNA expression level. *: p < 0.05 vs. None, #: p < 0.05 vs. LA2, and †: p < 0.05 vs. LA10 (Tukey’s HSD post hoc test, ANOVA); (B) Lipid accumulation in 3T3-L1 cells was assessed by oil red O staining. Differentiated adipocytes were visualized under a microscope. Abbreviations: Undiff, Undifferentiated; None, non-treated.