Table 2.
The effect of Cu supplementation in three doses (100%, 50%, and 10% of the requirement) and two forms (nanonaparticles and standard) into hen turkey diet on the AlaP, LeuAP, and ArgAP activity levels (mean ± SE) in breast and thigh muscle.
| Breast Muscle | Thigh Muscle | |||||
|---|---|---|---|---|---|---|
| Group * | AlaAP | LeuAP | ArgAP | AlaAP | LeuAP | ArgAP |
| 100S | 658.4 a ± 17.8 | 133.4 b,c ± 4.8 | 402.8 b,c ± 14.2 | 556.4 c,d ± 22.5 | 119.4 b ± 5.9 | 387.5 c,d ± 15.4 |
| 100N | 411.2 b ± 27.7 | 93.2 d ± 6.6 | 354.3 c ± 19.6 | 510.4 c,d ± 11.9 | 147.7 b ± 4.9 | 378.8 c,d ± 12.9 |
| 50S | 680.9 a ± 36.7 | 114.2 c,d ± 4.9 | 500.5 a ± 14.9 | 712.6 b ± 8.7 | 132.4 b ± 4.5 | 417.2 c ± 13.7 |
| 50N | 628.9 a ± 32.2 | 164.4 a,b ± 12.4 | 413.5b c ± 25.3 | 602.2 c ± 44.8 | 74.6 c ± 3.9 | 327.8 d ± 13.0 |
| 10S | 599.9 a ± 16.1 | 197.4 a ± 6.5 | 450.7 a,b ± 13.9 | 461.1 d ± 22.6 | 90.5 c ± 8.9 | 576.8 b ± 24.9 |
| 10N | 698 a ± 9.7 | 179.9 a ± 13.8 | 428.9 a,b ± 9.7 | 877.7 a ± 20.2 | 201.0 a ± 10.1 | 719.1 a ± 33.0 |
| Suppl. form effect | ||||||
| Nanoparticles | 579.4 b ± 29 | 145.9 ± 10 | 398.9 b ± 12.6 | 666.1 a ± 37.1 | 143.9 a ± 11.5 | 481.7 ± 39.2 |
| Standard | 646.4 a ± 15.7 | 148.3 ± 8 | 451.3 a ± 11.5 | 576.7 b ± 24.0 | 114.1 b ± 5.2 | 460.5 ± 20.2 |
| Dose effect | ||||||
| 100% | 534.8 b ± 35.7 | 113.3 c ± 6.5 | 378.6 b ± 13.3 | 533.4 b ± 13.6 | 133.6 a ± 5.2 | 383.2 b ± 9.8 |
| 50% | 654.9 a ± 24.5 | 139.3 b ± 9.1 | 456.9 a ± 18.1 | 661.1 a ± 25.2 | 105.4 b ± 8.2 | 375.5 b ± 15.0 |
| 10% | 648.9 a ± 15.6 | 188.7 a ± 7.7 | 439.8 a ± 8.7 | 669.4 a ± 55.7 | 145.7 a ± 15.7 | 647.9 a ± 27.1 |
| p-value | ||||||
| Source of variation | ||||||
| Suppl. Form | 0.0023 | 0.74 | 0.0005 | <0.001 | <0.001 | 0.38 |
| Dose | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 |
| Suppl. form × Dose | <0.001 | <0.001 | 0.17 | <0.001 | <0.001 | <0.001 |
* 100S: 100% of the dose in a standard form; 100N: 100% of the dose in a nanoparticle form; 50S: 50% of the dose in a standard form; 50N: 50% of the dose in a nanoparticle form; 10S: 10% of the dose in a standard form; 10N: 10% of the dose in a nanoparticle form; a–d Means in the same column with different letters are significantly different (p < 0.05) separately between the group, between the species and between the additives.