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The effect of substrate concentration on enzyme activity. Increasing concentrations of l-leucine (substrate) were reacted with purified l-amino acid oxidase. The average velocity was then plotted against the substrate concentration using a non-linear regression fit Michaelis-Menten model to describe the data. The values for Vmax and Km were calculated using GraphPad Prism 7 software (version 7.0, Graphpad Software, La Jolla, CA, USA).
