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. 2018 Jun 8;23(6):1388. doi: 10.3390/molecules23061388

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Percentage of viable, apoptotic, and necrotic cells in C. purpureomaculatus LAAO-treated SW480 (A–C) and SW620 (D–F) cells. Cells were treated with CP-LAAO for 24, 48, and 72 h. Treated and untreated cells (control) were double stained with annexin-V and propidium iodide and a minimum of 200 cells were counted per sample and the percentage of cells from each population (viable, apoptotic, and necrotic) was calculated. Experiments were performed in duplicates and comparable results were obtained from three independent experiments (n = 3). Comparison between data sets were performed using One Sample t-tests and asterisks indicate statistically significant (** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) differences between data sets for each treatment dose. No data were obtained for SW480 and SW620 treatment with 26 µg/mL of CP-LAAO at 72 h.

Percentage of viable, apoptotic, and necrotic cells in C. purpureomaculatus LAAO-treated SW480 (A–C) and SW620 (D–F) cells. Cells were treated with CP-LAAO for 24, 48, and 72 h. Treated and untreated cells (control) were double stained with annexin-V and propidium iodide and a minimum of 200 cells were counted per sample and the percentage of cells from each population (viable, apoptotic, and necrotic) was calculated. Experiments were performed in duplicates and comparable results were obtained from three independent experiments (n = 3). Comparison between data sets were performed using One Sample t-tests and asterisks indicate statistically significant (** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001) differences between data sets for each treatment dose. No data were obtained for SW480 and SW620 treatment with 26 µg/mL of CP-LAAO at 72 h.