Chemicals	Quantities
0.2 N HCl
1 N HCl	5 mL
Distilled water	20 mL
0.15 M Sodium cacodylate buffer stock
Sodium cacodylate	32.1 g [3H2O]
0.2 N HCl	25 mL
Make up 1 L with distilled water
pH 7.4
0.3 M Sodium cacodylate buffer stock
Sodium cacodylate	64.2 g [3H2O]
0.2 N HCl	25 mL
Make up 1 L with distilled water
pH 7.4
Tyrode Solution Composition
NaCl	139 mM
KCl	3 mM
CaCl2	3 mM
MgCl2	1 mM
Glucose	12 mM
NaHCO3	17 mM
Probenecid	1 mM
BDM	20 mM
NaCl/KCl/NaHCO3 1 L Solution
NaCl (Molecular Weight 58.44 g/mol)	81.2 g
KCl (Molecular Weight 74.55 g/mol)	2.24 g
NaHCO3 (Molecular Weight 84.01 g/mol)	14.28 g
Dissolve in 1 L distilled water
MgCl2/CaCl2 1 L solution
CaCl2 (2H2O) (Molecular Weight 147 g/mol)	1.764 g
MgCl2 (6H2O) (203.31 g/mol)	0.814 g
Dissolve in 1 L distilled water
1 M glucose 200 mL solution
Dextrose (Molecular Weight 180.16 g/mol)	36.03 g
Dissolve in 200 mL double distilled water
Tyrode Solution 500 mL solution
NaCl/KCl/NaHCO3	50 mL
Glucose	6 mL
Double distilled water	400 mL
Bubble oxygen in solution for 5 min
2% PFA+ 2.5% Glutaraldehyde +0.2% tanic acid fixative in 0.15 M sodium cacodylate
Paraformaldehyde powder (PFA)	2 g
Tanic acid	0.2 g
Distilled water	20 mL
25% Glutaraldehyde	10 mL
0.3 M sodium cacodylate	20 mL
0.15 M sodium cacodylate	50 mL
1 N NaOH	4 g NaOH/100 mL distilled water
4% Uranyl Acetate Stock Solution
Uranyl Acetate	4 g
Near boiling double distilled water	96 mL
Dissolve UA in near-bioling water using a stirrer in a fumehood.
Filter UA through a Whatman #1 filter into 200 mL brown bottle for light protection
Cap tightly and label, store at 4 °C