Figure 2. TRPA1 Increases [Ca²⁺]_i and Cell Survival in Response to H₂O₂ in Cancer Cells.

(A) Representative time courses and Δ[Ca²⁺]_i evoked by 10 μM H₂O₂ in the indicated HCC1569 cells. Data are shown as means ± SEM (n = 35–58). ***p < 0.001 compared with shGFP (Student’s t test).

(B and C) Δ[Ca²⁺]_i evoked by 10 μM H₂O₂(B) or 30 μM H₂O₂ (C) in the indicated lung cancer (B) and MPNST cells (C) treated with or without 10 μM AP-18. Data are shown as means ± SEM (n = 21–120 for B, n = 28–107 for C). *p < 0.05 and ***p < 0.001 (Student’s t test).

(D) Live cell numbers relative to H₂O₂-untreated cells in the indicated HCC1569 cells upon treatment with H₂O₂ for 96 hr. ***p < 0.001 compared with shGFP (Student’s t test).

(E) Percentage of cell death in the indicated HCC1569 cells treated with or without 10 μM H₂O₂ for 96 hr. ***p < 0.001 (Student’s t test).

(F) Live cell numbers relative to H₂O₂-untreated cells in EL-12-58 cells treated with H₂O₂ for 72 hr in the presence or absence of 10 μM AP-18. **p < 0.01 and ***p < 0.001 compared with vehicle control for AP-18 (AP-18 [–]) (Student’s t test).

(G) Percentage of cell death in EL-12-58 cells treated with or without 20 μM H₂O₂ for 72 hr in the presence or absence of 10 μM AP-18. ***p < 0.001 (Student’s t test).

(H–J) Live cell numbers relative to H₂O₂-untreated cells in the indicated cancer cells upon treatment with H₂O₂ for 72 hr. In (H and I), cells were treated with or without 10 μM AP-18. In (J), cells were transduced with an empty lentiviral vector or TRPA1. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with vehicle control for AP-18 (AP-18 [–]) in (H) and (I) and vector in (J) (Student’s t test).

(K) Live cell numbers relative to H₂O₂-untreated cells in the indicated HCC1569 cells treated with 8 μM H₂O₂ in the presence or absence of 10 μM BAPTA-AM for 96 hr. ***p < 0.001 (one-way ANOVA).

In (D–K), data are shown as means ± SD from the sum of three independent experiments performed in duplicate. See also Figure S2.