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. 2001 Jan;125(1):266–277. doi: 10.1104/pp.125.1.266

Figure 1.

Figure 1

BVR protein and enzyme levels in soluble protein extracts from WT and transgenic M-BVR seedlings. A, Immunblot analysis. Transgenic M-BVR (denoted p1–p6 and c1–c6) and wild-type (MMWT) seedlings were grown at 25°C on phytagel medium containing 1% (w/v) Suc for 10 d under Wc illumination of 130 μmol m−2 s−1. Whole seedling soluble protein extracts (20 μg) were used for BVR immunoblot analysis with anti-BVR antibody as described in “Materials and Methods.” A mature BVR standard (Mr approximately 33 kDa) is shown in the far right lane. B, Relative BVR Activity. Seedlings were grown on phytagel medium containing 1% (w/v) Suc for 16 d at 25°C under continuous Gro-lux/Gro-lux Wide Spectrum illumination of 130 μmol m−2 s−1. Whole seedling soluble protein extracts were used for BVR assays as described in “Materials and Methods.” Values were calculated relative to the specific activity for M-pBVR6, which was determined to be 2.86 × 10−3 IU mg−1 protein.