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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: Nature. 2018 Feb 7;555(7694):103–106. doi: 10.1038/nature25744

Figure 1. Piezo+ cells are EE precursors in the fly midgut.

Figure 1.

a. Piezo+ cells are esg-GFP and Dl-lacZ positive, but Su(H)Gbe-lacZ negative. b. Percentage of Piezo+ cells in esg+ cells. For Dl+: n=238 (Dl+), n=457 (esg+). For Piezo+: n=151 (Piezo+), n=682 (esg+). c. “newborn” EEs (arrowheads) are Piezo+. d. Piezo+ cells (RFP+) generate GFP+ EEs (arrowhead). e. Statistics of GFP+ ECs and EEs using Piezo-Gal4, Su(H)Gbe-Gal4, and Dl-Gal4. Number of cells analyzed: n=561 (Dl), n=432 (Su(H)), n=90 (Piezo). f. Bleomycin and DAPT treatment increase esg+, EP and EE cell numbers. Areas quantified: n=23 (Ctl), n=21 (Bleo), n=32 (DAPT). g,h. Elimination of Piezo+ cells by conditional expression of Rpr. Areas quantified: n=27 (Ctl), n=29 (Rpr), n=27 (Recover). i. pH3 staining of mitotic EPs (arrowhead). Data are expressed as mean + s.e.m. P-values are from two-tailed t-test. Scale bar: a, 20 μm; c,d,i, 10 μm; g, 50 μm.