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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: Kidney Int. 2016 May 21;90(1):135–148. doi: 10.1016/j.kint.2016.03.026

Figure 4. Long-term treatment with ouabain attenuates apoptosis of renal cortical cells in the PHN rat.

Figure 4.

A) Representative DAPI staining of nuclei in renal cortex of control rats, PHN rats and ouabain-treated PHN rats at four months after PHN induction. The arrows indicate typical condensed nuclei. For quantification control was set to 100%. All scale bars = 40μm.

B) Representative immunostaining for Bcl-xL in renal cortex of control rats, PHN rats and ouabain-treated PHN rats at four months after PHN induction. For semi-quantitative evaluation of Bcl-xL immunoreactivity signal, control was set to 100%. All scale bars = 40μm.

C) Representative immunostaining for Bax in renal cortex of control rats, PHN rats and ouabain-treated PHN rats at four months after PHN induction. For semi-quantitative evaluation of Bax immunoreactivity signal, control was set to 100%. All scale bars = 40μm.

For all experiments, analysis was done in two sections from each kidney and in five (condensed nuclei) or six (Bcl-xl and Bax) randomly selected areas of the outer cortex. Histograms show the mean ± SEM. Statistical analysis was performed using ANOVA followed by t-test. *p<0.05