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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: Kidney Int. 2016 May 21;90(1):135–148. doi: 10.1016/j.kint.2016.03.026

Figure 6. Kidneys from ouabain-treated PHN rats have more preserved WT1 positive glomerular cells than kidneys from vehicle-treated PHN rats.

Figure 6.

A) Representative micrographs showing TUNEL stain in WT1 positive glomerular cells from control rats, vehicle-treated and ouabain-treated PHN rats. Sections were TUNEL-stained (red) to detect apoptotic cells and stained for WT1 (green) to detect podocytes, DAPI stained nuclei are shown in blue. All scale bars = 20μm. Histogram shows quantification of apoptotic podocytes, for control rats, PHN rats and ouabain-treated PHN rats. Histograms show mean ± SEM.

B) Histogram shows quantification of WT1 positive cells per glomerulus for control rats, PHN rats and ouabain-treated PHN rats. Histograms show mean ± SEM.

For all experiments Mann-Whitney U test was used to determine significant differences *p<0.01, #p<0.05