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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Cancer Res. 2017 Sep 28;16(1):58–68. doi: 10.1158/1541-7786.MCR-17-0408

Figure 1. Inhibition of Hsp70 Causes Rapid Anti-Proliferative Activity.

Figure 1

(A) Chemical structures of Hsp70 inhibitors (JG-98, JG-84, MAL3-101 and VER-155008), controls (JG-258) and inhibitors of Hsp90 (17-DMAG) and the proteasome (bortezomib). (B) JG-98 (5 μM) suppress growth of MDA-MB-231 cancer cells with relatively rapid kinetics compared to 17-DMAG (5 μM) or bortezomib (40 nM), as monitored by MTT assays. Results are the average of two independent experiments performed in quintuplicates. The error bars represent the standard error of the mean (SEM). (C) Hsp70 inhibitors induce rapid cell growth effects, as measured by Cell Titer Glo. Results are the average of experiments performed in triplicate. Errors are SEM. (D) Chaperone clients are degraded relatively late after treatment with JG-98 (10 μM), after onset of effects on cell growth. Results are representative of experiments performed in duplicate.