Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Cancer Res. 2017 Sep 28;16(1):58–68. doi: 10.1158/1541-7786.MCR-17-0408

Figure 3. Inhibition of Caspases with z-VAD-fmk Does not Block JG-98’s Activity.

Figure 3

(A) Inhibition of caspases by z-VAD.fmk does not suppress the activity of JG-98. Results are the average of three independent experiments performed in quintuplicate. Error bars represent SEM. Cells were pretreated with z-VAD.fmk (40 μM) for 1 hour prior to addition of compounds (same concentrations as in panel A) and MTT assays performed after 24 hrs. **p value < 0.01. ns = not significant. Error is SEM. (B) Cells were treated as in panel A and visualized using an Olympus IX83 Inverted Microscope. Black arrows indicate apoptotic cells; white arrows indicate necrotic cells. (C) Hoechst 33258 staining of MDA-MB-231 cells pretreated with z-VAD.fmk (40 μM) for 1 hour prior to addition of JG-98 (10 μM) for 24 hours. Cells pre-treated with z-VAD.fmk show no nuclear fragmentation (white arrows), even after co-administration of JG-98. Scale bar is 10 μm.