| Reagent | Volume | Final Concentration |
| ddH2O | 6.3 μL | ---- |
| KCl (4M) | 0.94 μL | 300 mM |
| HEPES (0.5M) pH 7.4 | 0.5 μL | 20 mM |
| pCFJ90 (25 ng/μL) | 1.25 μL | 2.5 ng/μL |
| ssODN (500 ng/μL) | 1.25 μL | 50 ng/μL |
| sgRNA (50 μM) | 1.25 μL | 5 μM |
| Cas9 (61 μM) | 1.03 μL | 5 μM |
| Final Volume | 12.5 μL | ---- |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.