| Reagent | 1x | 24x |
| 2X Q5 Mastermix | 12.5 μL | 300 μL |
| Primer F1 (10 μM) | 1.25 μL | 30 μL |
| Primer R1 (10 μM) | 1.25 μL | 30 μL |
| Worm Lysis | 4 μL | ---- |
| ddH2O | 6 μL | 144 μL |
| Final Volume | 25 μL | 504 μL |
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