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(A-C) Lysates from U2OS cells transfected with NT or HERC1 siRNAs were analyzed by immunoblotting with antibodies against the indicated proteins. Levels of RAF proteins were quantified and normalized with respect to CHC levels (n = 4). Data are expressed as mean ± S.E.M. Statistical analysis was carried out as described under “Materials and Methods”. The differences are shown with respect to NT siRNA. ***, p< 0.001.
