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HeLa cells were transfected with the indicated siRNA (NT: non-targeting, Q1: HERC1, A: A-RAF, B: B-RAF, and C: C-RAF). (A) Colony formation assay was performed as indicated in “Materials and Methods” and cells were stained with crystal violet. (B) Crystal violet absorbance was quantified (n = 3). Data are expressed as mean ± S.E.M. Statistical analysis was carried out as described under “Materials and Methods”. *, p< 0.05.
