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. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: ACS Chem Biol. 2014 Jul 17;9(9):2111–2120. doi: 10.1021/cb5004064

Figure 3. SERCA inhibition with CPA and ER calcium depletion with has no effect on cytosolic free Zn2+ in primary murine β-cells and primary rat cardiomyocytes.

Figure 3

(a,b) Primary β-cells expressing eCALWY-4 were preincubated with Fura-2 (3µM) in KHB for 20 minutes before acquisition. Acquisitions rate was 12 images per minutes and fluorescence intensity ratios were determined sequentially for eCALWY-4 and Fura-2. Representative results are shown (a) Cells were perifused with KHB and after 3 minutes were treated with CPA 20 µM (n = 3 cells). (b) Cells were perifused with KHB containing 100 µM EGTA (Calcium-free buffer) and treated in the same conditions (n = 7 cells). (c) Cardiomyocytes expressing eCALWY-4 were perfused with KHB for 3 minutes then with KHB containing 20 µM CPA (n = 4 cells). Data are means ± S.E.M.