Differentiation of iPSCs (MRC5I & MRC9G) and ESCs (H1, H7 and H9) into definitive endoderm. A) qRT-PCR for definitive endoderm genes: SOX17, CXCR4, CER and FOXA2; B) Immunocytochemistry for SOX17 (10x magnification, scale bar 100um). N = 3, ANOVA with Fisher’s a priori test used to determine statistical significance of differences between groups.