Table 1. Top 10 miRNAs most highly up- (upper table) or down- (lower table) regulated during differentiation of ESCs and iPSCs to definitive endoderm.

Individual comparisons were carried out for the ESC lines (H1 & H9) as these clearly demonstrated distinct profiles of miRNA expression in the principal component analysis and hierarchical clustering analysis. P-values of <0.1 were considered statistically significant.

miRNAs Upregulated In DE Formation
miRNA	ESC p-value	iPSC p-value	H9 p-value	H1 p-value
hsa-miR-375	0.019	0.042	0.092	0.048
hsa-miR-708-5p	0.050	0.000	0.020	0.083
hsa-miR-744-5p	0.029	0.038	0.234	0.084
hsa-miR-27b-3p	0.036	0.070	0.085	0.084
hsa-miR-26b-5p	0.021	0.066	0.092	0.084
hsa-miR-30b-5p	0.050	0.068	0.108	0.104
hsa-miR-4530	0.019	0.051	0.155	0.109
hsa-miR-151a-3p	0.094	0.019	0.106	0.084
hsa-miR-151a-5p	0.066	0.020	0.165	0.084
hsa-miR-191-5p	0.023	0.081	0.273	0.084
miRNAs Downregulated In DE Formation
miRNA	ESC p-value	iPSC p-value	H9 p-value	H1 p-value
hsa-miR-3941	0.065	0.042	0.236	0.077
hsa-miR-3148	0.066	0.041	0.250	0.104
hsa-miR-124-5p	0.015	0.063	0.249	0.084
hsa-miR-4285	0.045	0.066	0.979	0.083
hsa-miR-3935	0.050	0.128	0.218	0.089
hsa-miR-378a-3p	0.021	0.313	0.086	0.075
hsa-miR-4451	0.035	0.163	0.015	0.148
hsa-miR-516b-5p	0.039	0.145	0.072	0.088
hsa-miR-4436b-5p	0.269	0.037	0.310	0.083
hsa-miR-4732-3p	0.380	0.005	0.952	0.083