Skip to main content
. Author manuscript; available in PMC: 2018 Aug 20.
Published in final edited form as: Diabetes. 2016 Feb 9;65(5):1268–1282. doi: 10.2337/db15-1240

Figure 8. Over-expression of Acot7 in β-cells results in reduced fatty-acyl-CoA generation but not fatty acid levels.

Figure 8

A) Total lyso-phosphatidic acid (LPA), phosphatidic acid (PA), lyso-phosphatidylcholine (LPC), acyl-linked phosphatidycholine (aPC), phosphatidycholine (PC), acyl-lyso-phosphatidylethanolamine (aLPE), lyso-phosphatidylethanolamine (LPE), acyl-phosphatidylethanolamine (aPE), phosphatidylethanolamine (PE), lyso-phosphatidylglycerol (LPG), phosphatidylglycerol (PG), lyso-phosphatidylinositol (LPI), phosphatidylinositol (PI), lyso-phosphatidylserine (LPS), phosphatidylserine (LPS), cardiolipin (CL), free fatty acids (FFA), fatty acyl CoA (FaCoA), fatty acyl carnitine (FaCN), monoacylglycerol (MG), diacylglycerol (DG), triacylglycerol (TG), sphingosine (SG), sphindogsine-1-phosphate (S1P), ceramide (Cer), sphingomyelin (SM), were analysed in isolated islets from 9-week old Acot7 Tg (green) or control littermantes (C, black) fed on a high fat diet for 4 weeks. B, C, D) Detailed free fatty acid (FA, (B)), fatty acyl-CoA (FaCoA, (C)) and fatty acyl-carnitine (FaCN, (D)) content in Acot7 Tg and control islets. n=3-5 mice/genotype, 150-350 islets/mice. Mice (male) were 9 week old and kept on high fat diet for 4 weeks. Isolated islets were pre-incubated at 3mM glucose during 1h and stimulated with 17mM glucose during 30 min prior snap-freezing. Data represent mean values ± S.E.M. *p<0.05; Unpaired Student’s t test.