| Temperature | Time | Ramp | Cycle(s) |
| 94oC | 2 min | 1 cycle | |
| 94oC | 20 s | 10 cycles | |
| 70oC | 1 s | ||
| 55oC | 30 s | 0.1 oC/s | |
| 68oC | 2:30 min | 0.2 oC/s | |
| 94oC | 20 s | 5 cycles | |
| 70oC | 1 s | ||
| 55oC | 30 s | 0.1 oC/s | |
| 68oC | 2:30 min | 0.2 oC/s + 5 s/cycle | |
| 94oC | 20 s | 10 cycles | |
| 70oC | 1 s | ||
| 55oC | 30 s | 0.1 oC/s | |
| 68oC | 2:30 min | 0.2 oC/s + 20 s/cycle | |
| 72oC | 10 min | 1 cycle | |
| 8oC | Hold |
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