HOLDING ACSF		RECORDING ACSF		CUTTING ACSF
Chemical	C [mM]		Chemical	C [mM]		Chemical	C [mM]
NaCl	115		NaCl	115		Sucrose	208
KCl	2		KCl	2		KCl	2
KH2PO4	1.25		KH2PO4	1.25		KH2PO4	1.25
MgSO4	1.3		MgSO4	1		MgCl2	5
CaCl2	2		CaCl2	2		MgSO4	2
D-glucose	25		D-glucose	25		CaCl2	0.5
NaHCO3	26		NaHCO3	26		D-glucose	10
L-Ascorbic Acid	1		L-Ascorbic Acid	1		NaHCO3	26
						L-Ascorbic Acid	1
pH	7.4		pH	7.4		Pyruvic Acid	3
Osmolality	300 mOsm/Kg		Osmolality	300 mOsm/Kg
						pH	7.4
						Osmolality	300 mOsm/Kg