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. 2018 Jul 27;9(58):31214–31230. doi: 10.18632/oncotarget.25781

Figure 2. Transcription of APC-associated genes changes in APC-deficient cells and mouse adenomas with activated WNT signaling.

Figure 2

A list of 2,886 genes associated with genomic regions enriched by APC ChIP were compared with 1,379 transcripts altered in expression following APC silencing by siRNA in the same cell line (Panels A, B) and 1,535 transcripts altered in expression following both Apc loss and Ctnnb1 activation in mouse colon adenomas (Panel C). Forty-nine genes satisfied both screening criteria, including ten candidates depicted in Panels B and C, and two positive controls (AXIN2 and SP5, indicated with red arrows). Two other positive controls (also indicated with red arrows) failed to satisfy these screening criteria due to an insignificant change in gene expression in either the cell line model alone (MYC) or in both models (DKK1). RNA-seq data for these genes are included in Panels B (human cell line) and C (mouse adenomas). Error bars representing standard deviation are included only for conditions for which multiple samples were sequenced.